Biodegradation Kinetics of Four Substituted Chlorobenzoic Acids by Enterobacter aerogenes

ABSTRACT Enterobacter aerogenes is generally found in soil, sewage plants, and human gastrointestinal tract. Thus, this study was conducted to evaluate the ability of Enterobacter aerogenes to degrade four chlorobenzoic acid compounds (2-chlorobenzoic acid (2-CBA), 3-chlorobenzoic acid (3-CBA), 4-chlorobenzoic acid (4-CBA), and 3,4-dichlorobenzoic acid (3,4-dCBA)) in minimal salt medium. Enterobacter aerogenes was partially able to degrade and dechlorinate these CBAs at concentration of 3.5 mM within 72 h of incubation. According to Haldane single-substrate model, the values of maximum predicted growth rate (?max), half saturation constant (Ks), and inhibition constant (Ki) fell in the range of 0.2?0.8 h?1, 8?41 mM, and 5?53 mM, respectively. Based on the estimated values of both ?, a growth-associated constant, and ?, a non?growth-associated constant, the production of chloride was predominantly growth associated, since negligible values of the ? were determined. Haldane model gave a good prediction of the CBA substrate utilization and degradation, and was in a very good agreement with the experimental data. Because of the capability of Enterobacter aerogenes to utilize these aromatic compounds as carbon and energy sources, this microorganism can be a valuable and promising candidate for use in the biotreatment of wastewater and soil samples contaminated with mixtures of chlorobenzoates.